Research

  • Xiaoting Zhang, PhD
  • Associate Professor
  • Academic Office:
    Vontz Center for Molecular Studies
    3125 Eden Avenue
    ML 0521
    Cincinnati, OH 45267
  • Phone: 513-558-3017
  • Email: xiaoting.zhang@uc.edu

    Research/Clinical Interests:

  • Mechanisms of transcriptional and epigenetic regulation of gene expression in breast cancer and development of RNA-based nanotherapuetics.

    Education/Credentials:

  • Bachelor's Degree: Zhejiang University, 1993
  • Master's Degree: Fudan University, 1996
  • Doctoral Degree: The University of Iowa, 2001
  • Fellowship: Rockefeller University, 2007

Peer Reviewed Publications (in chronological order):

  • Zhang, W., Zhang, X., Zhang, Y. H., Strokes, D. C., & Naren, A. P. (2016). Lumacaftor/ivacaftor combination for cystic fibrosis patients homozygous for Phe508del-CFTR. . Drugs of Today (Barcelona, Spain : 1998) , 52 (4) , 229-37.
  • Leonard M and Zhang X (2016). First Therapeutic Aptamer: VEGF-Targeting Macugen . Aptamers: Tools For Targeted Nanotherapy and Molecular Imaging , Pan Stanford Publishing , Page 319-335..
  • Zhang Y, Leonard M, Shu Y, Yang Y, Shu D, Guo P, and Zhang X. (2016). Overcoming Tamoxifen Resistance of Human Breast Cancer by Targeted Gene Silencing Using Multifunctional pRNA Nanoparticles . ACS Nano 11(1):335-346. , DOI: 10.1021/acsnano.6b05910.
  • Leonard M, Zhang Y and Zhang X. (2015). Small non-coding RNAs and aptamers in diagnostics and therapeutics . Methods in Molecular Biology , 1296 , 225-33.
  • Leonard, M., Zhang, Y., & Zhang, X. (2015). Small non-coding RNAs and aptamers in diagnostics and therapeutics. . Methods in Molecular Biology (Clifton, N.J.) , 1296 , 225-33.
  • Czyzyk-Krzeska, M. F., & Zhang, X. (2014). MiR-155 at the heart of oncogenic pathways. . Oncogene , 33 (6) , 677-8.
  • Zhang L, Cui J, Leonard M, Nephew K, Li Y, & Zhang X (2013). Silencing MED1 Sensitizes Breast Cancer Cells to pure Anti-estrogen Fulvestrant Therapy in vitro and in vivo . PLoS ONE , 8(7): e70641.
  • Germer, K., Leonard, M., & Zhang, X. (2013). RNA aptamers and their therapeutic and diagnostic applications. . International Journal of Biochemistry and Molecular Biology , 4 (1) , 27-40.
  • Zhang, L., Cui, J., Leonard, M., Nephew, K., Li, Y., & Zhang, X. (2013). Silencing MED1 sensitizes breast cancer cells to pure anti-estrogen fulvestrant in vitro and in vivo. . PloS One , 8 (7) , e70641.
  • Cui J, Germer K, Wu T, Wang J, Luo J, Wang SC, Wang Q, and Zhang X (2012). Cross-talk Between HER2 and MED1 Regulates Tamoxifen Resistance of Human Breast Cancer Cells . Cancer Research , 72 (21) , 5625-34.
  • Cui, J., Germer, K., Wu, T., Wang, J., Luo, J., Wang, S., Wang, Q., & Zhang, X. (2012). Cross-talk between HER2 and MED1 regulates tamoxifen resistance of human breast cancer cells. . Cancer Research , 72 (21) , 5625-34.
  • Pfaff D, Waters J, Kahn I, Zhang X and Numan M. (2011). Estrogen receptor-initiated mechanisms causal to mammalian reproductive behaviors. . Endocrinology , 152 (4) , 1209-17.
  • Zhang D, Jiang P, Xu Q, and Zhang X (2011). ARGLU1 interacts with MED1 and is required for estrogen receptor-mediated transcription and breast cancer cell growth. . Journal of Biological Chemistry , 286(20):17746-54.
  • Chen Z, Zhang C, Wu D, Rorick A, Zhang X, and Wang Q (2011). Specific phosphorylation of coactivator Mediator 1coding for UBE2C locus looping leads to androgen receptor negative prostate cancer growth . EMBO J. , 30(12):2405-19.
  • Zhang, D., Jiang, P., Xu, Q., & Zhang, X. (2011). Arginine and glutamate-rich 1 (ARGLU1) interacts with mediator subunit 1 (MED1) and is required for estrogen receptor-mediated gene transcription and breast cancer cell growth. . The Journal of Biological Chemistry , 286 (20) , 17746-54.
  • Jiang, P., Hu, Q., Ito, M., Meyer, S., Waltz, S., Khan, S., Roeder, R. G., & Zhang, X. (2010). Key roles for MED1 LxxLL motifs in pubertal mammary gland development and luminal-cell differentiation . Proc Natl Acad Sci U S A , 107 (15 ) , 6765-70.
  • Chen, W., Zhang, X., Birsoy, K., & Roeder, R. G. (2010). A muscle-specific knockout implicates nuclear receptor coactivator MED1 in the regulation of glucose and energy metabolism . Proc Natl Acad Sci U S A , 107 (22 ) , 10196-201.
  • Jiang, P., Hu, Q., Ito, M., Meyer, S., Waltz, S., Khan, S., Roeder, R. G., & Zhang, X. (2010). Key roles for MED1 LxxLL motifs in pubertal mammary gland development and luminal-cell differentiation. . Proceedings of the National Academy of Sciences of the United States of America , 107 (15) , 6765-70.
  • Zhang, X., Leung, Y., & Ho, S. (2007). AP-2 regulates the transcription of estrogen receptor (ER)-beta by acting through a methylation hotspot of the 0N promoter in prostate cancer cells. . Oncogene , 26 (52) , 7346-54.
  • Stumpf M, Waskow C, Krötschel M, Essen D, Rodriguez P, Zhang X, Guyot B, Roeder RG and Borggrefe T. (2006). The Mediator Complex Functions as a Coactivator for GATA1 in Erythropoiesis via Subunit Med1/TRAP220 . Proc. Natl. Acad. Sci. , 103:18504-9.
  • Zhang, X., Krutchinsky, A., Fukuda, A., Chen, W., Yamamura, S., Chait, B. T., & Roeder, R. G. (2005). MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription . Mol Cell , 19 (1 ) , 89-100.
  • Zhang, X., Kolaczkowska, A., Devaux, F., Panwar, S. L., Hallstrom, T. C., Jacq, C., & Moye-Rowley, W. S. (2005). Transcriptional regulation by Lge1p requires a function independent of its role in histone H2B ubiquitination . J Biol Chem , 280 (4 ) , 2759-70.
  • Le Crom, S., Devaux, F., Marc, P., Zhang, X., Moye-Rowley, W. S., & Jacq, C. (2002). New insights into the pleiotropic drug resistance network from genome-wide characterization of the YRR1 transcription factor regulation system . Mol Cell Biol , 22 (8 ) , 2642-9.
  • Zhang, X., & Moye-Rowley, W. S. (2001). Saccharomyces cerevisiae multidrug resistance gene expression inversely correlates with the status of the F(0) component of the mitochondrial ATPase . J Biol Chem , 276 (51 ) , 47844-52.
  • Zhang, X., Cui, Z., Miyakawa, T., & Moye-Rowley, W. S. (2001). Cross-talk between transcriptional regulators of multidrug resistance in Saccharomyces cerevisiae . J Biol Chem , 276 (12 ) , 8812-9.
  • Zhang, X., De Micheli, M., Coleman, S. T., Sanglard, D., & Moye-Rowley, W. S. (2000). Analysis of the oxidative stress regulation of the Candida albicans transcription factor, Cap1p . Mol Microbiol , 36 (3 ) , 618-29.
  • Zhang, X., Ma, B., Shu, N., Chen, SZ, and Zhao, SY. (1997). Cloning the Gene Coding for Human Glial Cell Line-derived Neurotrophic Factor and its Expression in Escherichia Coli . Chinese Journal of Biotechnology , 13(4): 426-9.
  • Zhang, X., Chen, SZ, and Zhao, SY. (1996). The Gene Structure of Ciliary Neurotrophic Factor and its Receptor . Foreign Med. Sci. (Molecular Biology) , 18(5):225-9.

    Research Grants:

  • Breast Cancer Alliance . Masin Young Investigator Award, Completed.
  • Hormone Research Foundation. C.H. Li Memorial Scholar Award, Completed.
  • Susan G. Komen Breast Cancer Foundation . Postdoctoral Fellowship, Completed.
  • University of Cincinnati Cancer Center and the Department of Cancer and Cell Biology. Start-up, Active.
  • University of Cincinnati Cancer Center. Cancer Center Pilot Grant, PI, Completed.
  • American Heart Association. Beginning Grant-in-Aid, Completed.
  • Ride Cincinnati Foundation. Ride Cincinnati Award, Completed.
  • University of Cincinnati. CCTST Junior Investigator Award, Completed.
  • Ohio Cancer Research Associate. Seed Money, PI, Completed.
  • Department of Defense. BRCP Idea Award, PI, Completed.
  • Susan G. Komen For the Cure Foundation. Career Catalyst Award, PI, Active.
  • American Cancer Society. Research Scholar Grant, PI, Active.
  • 09BGIA2010011 American Heart Association - Great Rivers Affiliate. Novel Role of Transcriptional Coregulator MED1 in Muscle Metabolic Functions, PI, Closed.
  • SRS 007568 Ohio Cancer Research Associates. The ER/MED1 Axis and Mammary Stem/Progenitor Cells, PI, Closed.
  • KG110028 Susan G. Komen Breast Cancer Foundation. Crosstalk among MED1, ER and HER2: New Strategy to Overcome Endocrine Resistance in Breast Cancer, PI, Active.
  • RSG-12-268-01-CCG American Cancer Society - National Chapter. MED1: Molecular Mechanisms Regulating Cellular Senescence in Breast Cancer, PI, Active.
  • R01CA197865 National Institutes of Health. Role of MED1 in HER2-driven Breast Tumorigenesis, PI, Awarded.

Recent Awards/Honors:

  • Graduate Senator Travel Award. The University of Iowa, 2000
  • Masin Young Investigator Award. Breast Cancer Alliance, 2002
  • C.H. Li Memorial Scholar Award. Hormone Research Foundation, 2004
  • Postdoctoral Fellowship Award. Susan G. Komen Breast Cancer Foundation, 2005
  • Komen Mentorship Award. Susan G. Komen Breast Cancer Foundation, 2005
  • Ride Cincinnati Award. Ride Cincinnati Breast Cancer Foundation, 2010
  • IDEA award. Department of Defense BCRP, 2011
  • Career Catalyst Award . Susan G. Komen for the Cure Foundation, 2011
  • Research Scholar. American Cancer Society, 2012
  • Scientific Reviewer Panel, DOD Breast Cancer Research Program, 2014
  • Ad Hoc Reviewer, NIH Tumor Progression and Metastasis (TPM) Study Section, 2014
  • Grant Reviewer, Susan G. Komen for the Cure Foundation, 2014
  • Ad Hoc Reviewer. NIH Tumor Progression and Metastasis (TPM) Study Section, 2015
  • Ad Hoc Reviewer NIH Tumor Progression and Metastasis (TPM) Study Section, 2016
  • Ad Hoc Reviewer, NIH Cancer Molecular Pathobiology (CAMP) Study Section , 2016
  • 2016 Ad Hoc Reviewer, NIH Tumor Progression and Metastasis (TPM) Study Section
  • Scientific Reviewer Panel, DOD Breast Cancer Research Program, 2016
  • Ad Hoc Reviewer, American Cancer Society, 2017
  • Grant Reviewer, Susan G. Komen for the Cure Foundation, 2017