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Xiaoting Zhang, PhD
Assistant Professor, Department of Cancer Biology, UC College of Medicine
Vontz Center for Molecular Studies
B.S.: Zhejiang University, 1993
M.S.: Fudan University, 1996
Ph.D.: The University of Iowa, 2001
Postdoctoral Fellow: Rockefeller University, 2007
Peer Reviewed Publications (in chronological order):
M Czyzyk-krzeska and Zhang X (2013). MiR-155 at the Heart of Oncogenic Pathways . Oncogene , Advance online publication, 18 February 2013; doi:10.1038/onc.2013.26.
Germer K, Pi M, Guo P and Zhang X (2013). Conjugation of RNA aptamer to pRNA nanoparticles for RNA-based therapy . RNA Nanotechnology and Therapeutics , CRC Press (2013 July) , 399-408 .
Germer K and Zhang X (2013). RNA aptamer and its diagnostic and therapeutic applications. . Int J Biochem Mol Biol , 4(1):27-40.
Zhang L, Cui J, Leonard M, Nephew K, Li Y, and Zhang X (2013). Silencing MED1 Sensitizes Breast Cancer Cells to Anti-estrogen Fulvestrant Therapy in vitro and in vivo . PLoS ONE , 8(7): e70641. doi:10.1371/journal.pone.0070641.
Cui J, Germer K, Wu T, Wang J, Luo J, Wang SC, Wang Q, and Zhang X (2012). Crosstalk Between HER2 and MED1 Regulates Tamoxifen Resistance of Human Breast Cancer Cells . Cancer Research , Epub online doi: 10.1158/0008-5472 .
Pfaff D, Waters J, Kahn I, Zhang X and Numan M. (2011). Estrogen receptor-initiated mechanisms causal to mammalian reproductive behaviors. . Endocrinology , 152 (4) , 1209-17.
Zhang D, Jiang P, Xu Q, and Zhang X (2011). ARGLU1 interacts with MED1 and is required for estrogen receptor-mediated transcription and breast cancer cell growth. . Journal of Biological Chemistry , 286(20):17746-54.
Chen Z, Zhang C, Wu D, Rorick A, Zhang X, and Wang Q (2011). Specific phosphorylation of coactivator Mediator 1coding for UBE2C locus looping leads to androgen receptor negative prostate cancer growth . EMBO J. , 30(12):2405-19.
Jiang, P., Hu, Q., Ito, M., Meyer, S., Waltz, S., Khan, S., Roeder, R. G., & Zhang, X. (2010). Key roles for MED1 LxxLL motifs in pubertal mammary gland development and luminal-cell differentiation . Proc Natl Acad Sci U S A , 107 (15 ) , 6765-70.
Chen, W., Zhang, X., Birsoy, K., & Roeder, R. G. (2010). A muscle-specific knockout implicates nuclear receptor coactivator MED1 in the regulation of glucose and energy metabolism . Proc Natl Acad Sci U S A , 107 (22 ) , 10196-201.
Stumpf M, Waskow C, Krötschel M, Essen D, Rodriguez P, Zhang X, Guyot B, Roeder RG and Borggrefe T. (2006). The Mediator Complex Functions as a Coactivator for GATA1 in Erythropoiesis via Subunit Med1/TRAP220 . Proc. Natl. Acad. Sci. , 103:18504-9.
Zhang, X., Krutchinsky, A., Fukuda, A., Chen, W., Yamamura, S., Chait, B. T., & Roeder, R. G. (2005). MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription . Mol Cell , 19 (1 ) , 89-100.
Zhang, X., Kolaczkowska, A., Devaux, F., Panwar, S. L., Hallstrom, T. C., Jacq, C., & Moye-Rowley, W. S. (2005). Transcriptional regulation by Lge1p requires a function independent of its role in histone H2B ubiquitination . J Biol Chem , 280 (4 ) , 2759-70.
Le Crom, S., Devaux, F., Marc, P., Zhang, X., Moye-Rowley, W. S., & Jacq, C. (2002). New insights into the pleiotropic drug resistance network from genome-wide characterization of the YRR1 transcription factor regulation system . Mol Cell Biol , 22 (8 ) , 2642-9.
Zhang, X., & Moye-Rowley, W. S. (2001). Saccharomyces cerevisiae multidrug resistance gene expression inversely correlates with the status of the F(0) component of the mitochondrial ATPase . J Biol Chem , 276 (51 ) , 47844-52.
Zhang, X., Cui, Z., Miyakawa, T., & Moye-Rowley, W. S. (2001). Cross-talk between transcriptional regulators of multidrug resistance in Saccharomyces cerevisiae . J Biol Chem , 276 (12 ) , 8812-9.
Zhang, X., De Micheli, M., Coleman, S. T., Sanglard, D., & Moye-Rowley, W. S. (2000). Analysis of the oxidative stress regulation of the Candida albicans transcription factor, Cap1p . Mol Microbiol , 36 (3 ) , 618-29.
Zhang, X., Ma, B., Shu, N., Chen, SZ, and Zhao, SY. (1997). Cloning the Gene Coding for Human Glial Cell Line-derived Neurotrophic Factor and its Expression in Escherichia Coli . Chinese Journal of Biotechnology , 13(4): 426-9.
Zhang, X., Chen, SZ, and Zhao, SY. (1996). The Gene Structure of Ciliary Neurotrophic Factor and its Receptor . Foreign Med. Sci. (Molecular Biology) , 18(5):225-9.
Breast Cancer Alliance . Masin Young Investigator Award, Completed.
Hormone Research Foundation. C.H. Li Memorial Scholar Award, Completed.
Susan G. Komen Breast Cancer Foundation . Postdoctoral Fellowship, Completed.
University of Cincinnati Cancer Center and the Department of Cancer and Cell Biology. Start-up, Active.
University of Cincinnati Cancer Center. Cancer Center Pilot Grant, PI, Completed.
American Heart Association. Beginning Grant-in-Aid, Completed.
Ride Cincinnati Foundation. Ride Cincinnati Award, Completed.
University of Cincinnati. CCTST Junior Investigator Award, Completed.
Ohio Cancer Research Associate. Seed Money, PI, Active.
Department of Defense. BRCP Idea Award, PI, Active.
Susan G. Komen For the Cure Foundation. Career Catalyst Award, PI, Active.
American Cancer Society. Research Scholar Grant, PI, Active.
09BGIA2010011 American Heart Association - Great Rivers Affiliate. Novel Role of Transcriptional Coregulator MED1 in Muscle Metabolic Functions, PI, Closed.
W81XWH-11-1-0118 Department of the Army. Targeting MED1 LxxLL Motifs for Tissue Selective Treatment of Human Breast Cancer, PI, Active.
SRS 007568 Ohio Cancer Research Associates. The ER/MED1 Axis and Mammary Stem/Progenitor Cells, PI, Active.
KG110028 Susan G. Komen Breast Cancer Foundation. Crosstalk among MED1, ER and HER2: New Strategy to Overcome Endocrine Resistance in Breast Cancer, PI, Active.
RSG-12-268-01-CCG American Cancer Society - National Chapter. MED1: Molecular Mechanisms Regulating Cellular Senescence in Breast Cancer, PI, Active.
Graduate Senator Travel Award. The University of Iowa, 2000
Masin Young Investigator Award. Breast Cancer Alliance, 2002
C.H. Li Memorial Scholar Award. Hormone Research Foundation, 2004
Postdoctoral Fellowship Award. Susan G. Komen Breast Cancer Foundation, 2005
Komen Mentorship Award. Susan G. Komen Breast Cancer Foundation, 2005
Ride Cincinnati Award. Ride Cincinnati Breast Cancer Foundation, 2010
IDEA award. Department of Defense BCRP, 2011
Career Catalyst Award . Susan G. Komen for the Cure Foundation, 2011
Research Scholar. American Cancer Society, 2012
Our laboratory aims to elucidate the molecular mechanism of gene transcriptional regulation and its desregulation in breast cancer. Breast cancer is the most common type of cancer and one of the leading causes of death among Western women. A vast majority (75%) of breast cancer has been found to express estrogen receptor (ER), which is the key mediator of estrogen functions and plays prominent roles in breast tumorigenesis and drug resistance. Anti-estrogens, such as tamoxifen, have been widely used in the treatment of ER-positive breast cancer, but acquired resistance and severe side effect on other estrogen-responsive tissues has greatly limited their usefulness. Hence, further development of novel strategies to selectively block estrogen signaling pathways is urgently needed. Our recent work has established MED1 (Mediator Subunit 1) as a key tissue-specific transcriptional coactivator in mediating estrogen receptor functions in both normal mammary gland development and breast cancer. Significantly, MED1 has been reported to be overexpressed or amplified in a high percentage (40% to 50%) of human breast cancers. We are currently continuing to determine the role of MED1 and its associated proteins in breast cancer by using a combination of state-of-art biochemical, molecular and cellular techniques, as well as mouse genetic approaches with our newly generated MED1 mutant knockin, conditional knockout and overexpression models. Most recently, we have also started developing RNA nanotechnology-based strategies to target MED1 to explore its therapeutic potentials. Through these investigations, our ultimate goal is to provide novel insights and potential therapeutic targets for better diagnosis, prognosis and individualized treatment of human breast cancer.
Xiaoting Zhang (08-2001). . Yeast Cell Biology Meeting , Cold Spring Harbor. Conference. International
Xiaoting Zhang (09-2005). . EMBO Conference: Nuclear receptors: from chromatin to disease , Lake Garda, Italy. Conference. International
Xiaoting Zhang (06-2006). . Komen Mission Conference: Many Faces – One Voice , Washington D.C. Conference. International
Xiaoting Zhang (06-2007). . Research Conference , Department of Surgery, University of Cincinnati. UC. University
Xiaoting Zhang (06-2007). . Department of Environmental Health , University of Cincinnati. UC. University
Xiaoting Zhang (07-2007). . Oncology Retreat , University of Cincinnati. UC. University
Xiaoting Zhang (10-2007). . UC Cancer Center Hormonal Malignancies Program , University of Cincinnati. UC. University
Xiaoting Zhang (01-2008). . Cancer and Cell Biology Seminar , Department of Cancer and Cell Biology. UC. University
(08-1998). Multiple Cys6 zinc cluster transcription factors regulates multidrug resistance in Saccharomyces cerevisiae.. Yeast Genetics and Molecular Biology Meeting , College Park, MD. Conference. International
(08-2000). Regulation and function of the zinc cluster transcription factor Yrr1p.. Yeast Genetics and Molecular Biology Meeting , Seattle, WA. Conference. International
(04-2001). Regulations of the transcriptome adaptations to cellular environment: the yeast multidrug resistance phenomenon.. The fifth annual international conference on computational molecular biology , Montreal, Canada. Conference. International
(08-2002). Ypl055cp functions downstream from Pdr3p during retrograde activation of PDR5 transcription. Yeast Genetics and Molecular Biology Meeting , Madison WI. Conference. International
(07-2005). MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation and is required for estrogen receptor-mediated transcription and breast cancer cell growth. 4th annual future of breast cancer conference , Fairmont hotel, Bermuda. Conference. International
(08-2005). MED1/TRAP220-containing TRAP/Mediator subpopulation and its role in estrogen receptor-mediated transcription.. Eukaryotic Transcription Mechanism , Cold Spring Harbor, NY. Conference. International
(07-2007). Role of transcriptional coactivator MED1/TRAP220 in glucose homeostasis and insulin sensitivity.. Digestive Health Center Research Forum: Bench-to-Bedside Research in Pediatric Digestive Disease , Cincinnati, OH. UC. University
(11-2007). Analyses of MED1/TRAP220 and Its Associated Protein Subunits. Annual Biomedical Research Conference for Minority Students (ABRCMS) , Austin, TX. Conference. National
Cancer and Cell Biology Graduate Program Annual Symposium , Symposium University of Cincinnati12-2007. College
Positions and Work Experience
1994-1996, Graduate Research Assistant, Institute of Genetics,Fudan University, Shanghai, P.R.China
1994-1995, Graduate Teaching Assistant, Institute of Genetics,Fudan University, Shanghai, P.R.China
1996-2001, Graduate Research Assistant, University of Iowa, Iowa City, IA
2002-2007, Postdoctoral Research Fellow, Rockefeller University, New York, NY
2007, Assistant Professor, University of Cincinnati, Cincinnati, OH
2007, Faculty Member, Graduate Program in Cell and Cancer Biology, University of Cincinnati, Cincinnati, OH
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